Phospho-4ebp1
WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the … WebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
Phospho-4ebp1
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WebTranslation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity ... WebThe Phospho-4EBP1 (Thr37/46) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific …
WebMar 22, 2024 · DRAM1 also dramatically downregulated the phosphorylation of both rpS6 at Ser 240/244 (Fig. 2 a and c) and 4EBP1 (Fig. 2 a and d), suggesting that the activity of p70S6K was decreased, though the phosphorylation of mTOR itself had no change (Fig. 2 a). Web运动和PI3K阻断剂对大鼠骨骼肌mTOR及下游信号的作用研究,sd大鼠,大鼠阅读答案,wistar大鼠,大鼠解剖图,大鼠翻译,大鼠 蒲松龄,大鼠十二脂肠插管,大鼠固定器,大鼠代谢笼
WebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 … WebJul 7, 2016 · GSK-3 differentially regulates 4EBP1 and S6RP phosphorylation in ACHN cells. a Changes in the phosphorylation levels of 4EBP1 and S6RP over time after treatment of AR-A014418 (25 μM) or rapamycin ...
WebThe aim of this study was to evaluate the role of phosphorylated 4EBP1 (p4EBP1) in primary renal cell carcinoma (RCC) as a biomarker in metastatic RCC (mRCC) and non-mRCC …
WebThe ER is the main organelle for calcium (Ca 2+) storage in cells.Its physiological function is closely related to Ca 2+ homeostasis (Wong et al., 1993).To investigate the effect of As 3+ /Se 4+ combination on ER function, this study pretreated cells with ERS inducer thapsigargin (TG) and ERS inhibitor 2-aminoethoxydiphenyl borate (2-APB), followed by Fluo-3-AM and … megadeth tornado of souls tempoWebPrimary Antibodies (9010) Secondary Antibodies (76) Antibody Conjugates (1994) Activators & Inhibitors (224) Buffers & Dyes (108) Cellular Assay Kits (118) ChIP Kits & Reagents (156) CUT&RUN Kits & Reagents (15) Cytokines & Growth Factors (215) ELISA Kits (513) Experimental Controls (248) Flow Cytometry Kits & Reagents (9) Proteomic Analysis … names that have shortenedWebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. … megadeth torontoWebPhospho-4EBP1 (Thr46) Antibody (44-1170G) in ICC/IF Immunofluorescence analysis of 4EBP1 (pT46) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. megadeth tickets hawaiiWebJan 9, 2012 · MPTECs were tranduced either with empty vector or NL4-3 and then incubated in media for 72 hours. Subsequently, proteins were extracted from vector/MPTECs and HIV/MPTECs, Western blots were prepared and probed for phospho-70S6K, phospho-eIF4B, and phospho-4EBP1. Immunoblots were stripped and reprobed for actin (n = 4). A. megadeth tornado of souls reaction youtubeWebImmunoprecipitation of Phospho-4E-BP1 (Ser65) from MCF-7 cell extracts. Cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then treated with 100 nM insulin for 30 minutes. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is ... megadeth to le mondeWebOur 4EBP1 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit and Mouse. These antibodies have been verified by … megadeth toronto 2021