Hcrt promoter

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August undefined, 2024

WebDec 12, 2024 · Hcrt-positive neurons are restricted to the perifornical hypothalamus (PFH) and lateral hypothalamic area (LHA) but have widespread projections to brain regions that differentially express HcrtR1 and HcrtR2, including dense projections to the noradrenergic areas of the locus coeruleus (LC), the nucleus accumbens, serotonergic areas of the … WebHCRT promoter (Faraco et al., 2006) flanked by NcoI/BamHI was am-plified and subcloned upstream to nfsB replacing the insulin promoter. To prepare probes for whole-mount ISH experiments, the full coding sequences of the following genes were amplified: hcrt (DQ831346.1), mch1 (FJ204827.1), hcrtr (EF150847.1), dat (NM_131755.1), d h

Evolutionarily conserved regulation of hypocretin neuron …

WebConstructs—Zebrafish hcrt promoter constructs 2kb-zhcrt-EGFP ( 1941 to 62) and 1kb-zhcrt-EGFP ( 941 to 62, GenBankTM DQ831347) fragments were amplified by PCR of genomic DNA and ligated into the SalI-BamH1 sites of pEGFP-1. A 1040-bp T. nigroviridis hcrt promoter fragment (1–1040 of GenBankTM DQ831348) was amplified from BAC WebThe boxed region is shown at higher magnification in the inset. (B) Schematic of the zebrafish 1 kb hcrt promoter, including putative Lhx9 binding sites A and B. (C) … nttデータ cm

Hypocretin/Orexin Overexpression Induces An Insomnia-Like …

WebOct 17, 2007 · The 3,086-base-pair ( Eco RI– Sac I) mouse Hcrt promoter 19, 20 was used to replace the CaMKIIα promoter in the CaMKIIα::ChR2-mCherry lentivirus vector 25. The Hcrt::mCherry control viruses... WebMar 15, 2015 · Loss of neurons that express the neuropeptide hypocretin (Hcrt) has been implicated in narcolepsy, a debilitating disorder characterized by excessive daytime … WebSep 25, 2012 · Current models of sleep/wake regulation posit that Hypocretin (Hcrt)-expressing neurons in the lateral hypothalamus promote and stabilize wakefulness by projecting to subcortical arousal centers. However, the critical downstream effectors of Hcrt neurons are unknown. agrilunassa eco guest house

Modular organization of a hypocretin gene minimal promoter

Neural substrates of awakening probed with …

WebHCRT neurotransmission promotes operant responding for both drug and non-drug rewards. ... and cannabis dependent subjects. Promoter methylation did not differ between … WebDec 22, 2009 · Hypocretin 1 and 2 (HCRT 1 and 2, also known as orexin A and B) are two neuropeptides originally isolated in rats, that are derived from a single gene precursor (Hcrt/Orx) (1, 2).HCRT preproprotein is exclusively expressed in neurons restricted to the lateral hypothalamus (LH) organized as a single compact cluster in each hemi-brain … agri-logisticsWebSep 24, 2024 · We show that the human HCRT gene promoter is methylation-sensitive in vitro, and is hypermethylated in the hypothalamus of patients selectively at a putative PAX5:ETS1 binding site within the proximal HCRT promoter. nttデータ ec

"WebSep 9, 2012 · We used the zebrafish 2-kilobasepair hcrt promoter to drive the expression of the nfsB-EGFP fusion protein specifically in HCRT neurons. This promoter was … " - Hcrt promoter

Hcrt promoter

Evolutionarily conserved regulation of hypocretin neuron …

WebThe hypocretin (Hcrt, also known as orexin) neuropeptides regulate sleep and wake stability, and disturbances of Hcrt can lead to sleep disorders. MicroRNAs (miRNAs) are short noncoding RNAs that fine-tune protein expression levels, and miRNA-based therapeutics are emerging. We report a functional interaction between miRNA (miR-137) … WebMar 15, 2015 · The boxed region is shown at higher magnification in the inset. (B) Schematic of the zebrafish 1 kb hcrt promoter, including putative Lhx9 binding sites A and B. (C) Sequence of a previously characterized mammalian Lhx9 binding site compared with sites A and B in the zebrafish hcrt promoter and in mutated constructs. Gray shading, …

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WebAbstract. Current models of sleep/wake regulation posit that Hypocretin (Hcrt)-expressing neurons in the lateral hypothalamus promote and stabilize wakefulness by projecting to … WebTargeting the Hcrt system with optogenetics. To genetically target Hcrt neurons, we stereotaxically inject a virus carrying ChR2 driven by the Hcrt promoter into the lateral …

WebApr 11, 2024 · Methylation of the HCRT and GAL promoters was not associated with patients with HNSCC or the normal control group. A Pearson correlation revealed … WebIn the present study, a minimal promoter region of the murine Hcrt gene was identified, which was able to drive the expression of a luciferase reporter gene in the human 293 cell line. Deletion of the olf1-site proximal to the transcription start site of the Hcrt gene increased reporter gene expression, whereas deletion of the distal olf1-like ...

WebAug 9, 2011 · Mice expressing ChR2 under the Hcrt promoter were stimulated with a blue laser diode (477 nm; 20 mW) through an optical fiber aimed at the lateral hypothalamus. Trains of 10 s (20 Hz, 15-ms light pulse) with 30- or 60-s intervals between the stimuli were used. ... Hcrt neurons are thought to participate in the homeostatic regulation of sleep by ... WebAug 10, 2007 · This important question can be investigated in a transgenic (TG) line of rats created to mimic the loss of HCRT neurons seen in human narcoleptics. In these rats, there is a gradual and selective loss of HCRT neurons as a result of a HCRT promoter-driven accumulation of the cytotoxic gene product poly-Q-ataxin-3 (Beuckmann et al., 2004).

WebDec 24, 2016 · The hypothalamic Hypocretin/Orexin (Hcrt) neurons secrete two Hcrt neuropeptides. These neurons and peptides play a major role in the regulation of feeding, sleep wake cycle, reward-seeking, addiction, and stress. Loss of Hcrt neurons causes the sleep disorder narcolepsy.

WebMar 21, 2024 · HSPB9 piR-30173 KAT2A DHX58 ZNF385C KCNH4 HCRT ENSG00000267261: GH17J042515: Promoter/Enhancer: 1.8 : FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER: 10.10: 17.73-336.4: 73: … agrilunassa bordigheraWebDec 20, 2006 · Our zebrafish model of Hcrt overexpression indicates that the ancestral function of Hcrt is to promote locomotion and inhibit rest and will facilitate the discovery of neural circuits, genes, and drugs that regulate Hcrt function and sleep. hypocretin orexin sleep insomnia circadian rhythm zebrafish Introduction a grill matWebApr 18, 2011 · Presence of olf-1 (O/E3 binding site) like elements in the promoter region of prepro-orexin genes. Alignment of the upstream (283–300 bp) sequences of the prepro-orexin (prepro-hypocretin) locus (Hcrt) from four separate mammals shows two highly conserved olf-1-like (olf-1/ebf, bold face) elements. agrima batra designerWebAug 10, 2007 · A transgenic (TG) rat model has been developed to mimic the neuronal loss found in narcoleptic humans. In these rats, HCRT neurons gradually die as a result of the expression of a poly-glutamine repeat under the control of the HCRT promoter. nttデータ mejar オープンWebSep 24, 2024 · We show that the human HCRT gene promoter is methylation-sensitive in vitro , and is hypermethylated in the hypothalamus of patients selectively at a putative … nttデータ gwサービスNational Center for Biotechnology Information nttデータ dx 戦略WebOct 1, 2010 · The earliest detected aminergic cells are found adjacent to the nucleus of the tract of the postoptic commissure, nTPOC, at 20–24 h post-fertilization (hpf). Both serotonergic and catecholaminergic cells are found at this time point in this region, a few cells even exhibiting double phenotype (tyrosine hydroxylase and serotonin as markers). agrima botanicals